基因工程
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        本实验课程是以DNA重组技术为主线,强调实验方法的经典性、实用性。实验内容涉及了基因工程的主要过程。以含有表达载体质粒和目的片段的宿主菌为出发菌株经碱法抽提得到质粒DNA,对其进行浓度及纯度的测定后,用限制性内切酶进行切割,回收供体和载体片断,将载体与供体连接,然后进行转化得到重组子,以PCR方法鉴定重组子。然后将重组质粒转入表达菌株中进行诱导表达,最后以SDS-PAGE的方法对产物进行鉴定。

        整个实验基本上是一个连续的过程,通过学习,要求学生能在原有的相关理论知识基础上,较全面和深入理解基因工程原理、基本掌握基因工程常用的实验方法,以求为以后的学习和科研工作打下良好和扎实的基础。


        The course focus on the DNA recombine technology and emphasize on the classic and practicability experiment. The content including the process of gene engineering.From extract the plasmid DNA and mensurate the thickness and purity of it, then cut by enzyme, recycle the objective DNA and joint them. Transport the recombined plasmid DNA to E.coli.Identify them by PCR. Induced expression the recombined plasmid and indentity the production by SDS-PAGE.

        The course is a successively experiment and required the students master the theory and technology about gene engineering.That benefit the future study for students.